Comparison Hirst/Coriolis® for Pollen Count Monalisa Project - Bertin Technologies

Comparison Hirst/Coriolis® for Pollen Count Monalisa Project

Context

Within the field of MONALISA European LIFE project developed to validate a new method for pollen and allergen detection, the innovative Coriolis® continuous cyclonic air sampler is compared with the usual Hirst pollen trap

Protocol

•HIRST sampling 04131-203-SL004 Fiche Monalisa
? Cylinder rotation 2 mm/h
? Air flow rate 10 L/min => Adhesive band
? Band recovered and cut out in 24h segments
? Segments coloured for microscopy
? Sample totality read by optical microscope x400
•CORIOLIS® sampling
?Air flow rate 200 l/min
?Sampling time 60 min => Liquid sample
?Centrifugation and elimination of the supernatant to keep a 2 ml residue
?Homogenization of the residue and preparation of 3 slides
?7 horizontal lines of the 3 slides read by optical microscope

04131-203-SL004 Fiche Monalisa 2

Results

Daily comparison with chi-square test for the total pollens, specific ones and fungi’s spores (Alternaria, Ambrosia, Artemisia, Betula, Cupressaceae, Parietaria / Urticaceae, Poaceae,)

04131-203-SL004 Fiche Monalisa 3 04131-203-SL004 Fiche Monalisa 4

Customer

Conclusion

The efficiency of Coriolis® and Hirst are both representative and equivalent.
The use of Coriolis® liquid sample gives access to immunological analyses to assess the allergenicity/antigenicity of the collected pollens

ANY QUESTIONS? CONTACT AN EXPERT

Please fill in this form if you wish to contact our sales team or receive further information.

My Account
Forgot password ?

Add a question !

Add a question