Context
Within the field of MONALISA European LIFE project developed to validate a new method for pollen and allergen detection, the innovative Coriolis® continuous cyclonic air sampler is compared with the usual Hirst pollen trap
Protocol
•HIRST sampling
? Cylinder rotation 2 mm/h
? Air flow rate 10 L/min => Adhesive band
? Band recovered and cut out in 24h segments
? Segments coloured for microscopy
? Sample totality read by optical microscope x400
•CORIOLIS® sampling
?Air flow rate 200 l/min
?Sampling time 60 min => Liquid sample
?Centrifugation and elimination of the supernatant to keep a 2 ml residue
?Homogenization of the residue and preparation of 3 slides
?7 horizontal lines of the 3 slides read by optical microscope
Results
Daily comparison with chi-square test for the total pollens, specific ones and fungi’s spores (Alternaria, Ambrosia, Artemisia, Betula, Cupressaceae, Parietaria / Urticaceae, Poaceae,)
Customer
