Choosing your lysing kit

The disruption of samples is the first step in the process of isolation and/or quantification of nucleic acids, proteins, and analytes. For this reason, choosing the right homogenization protocol will have a great impact on the quality and reliability of results for most biological analytical workflows.

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Bertin Technologies offers a range of robust, efficient, and flexible homogenizers and lysing kits. Our products are based on bead beating technology that can grind any tissues and lyse any cells prior to DNA, RNA, and protein analysis.

Homogenization protocols can vary significantly depending on the nature and the quantity of the sample. Our team of scientists have designed a comprehensive range of lysing kits that provide researchers with an optimized solution for each of their workflows.

When using the 3D bead-beating technology, the choice of the bead size and material can greatly affect the disruption energy applied to the sample. The optimal bead density and bead material vary depending on the sample hardness. For harder samples, the bead material should be more resistant. For example, seeds can be homogenized with metal beads. For a given sample hardness, the bead size should be proportional to the target size. For example, bacteria vs 0.1mm bead. Sometimes, mixing the bead sizes and/or material increases the efficiency of the homogenization.

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See Table 1 to identify a suitable kit for your sample

Name	Application	Bead type	Available tube volume
			Well-Plate	0.5mL	2mL	2mL R*	7mL	15mL
VK01	Micro-organism lysing	0.1mm glass beads			X
VK05	Tough micro-organism lysing	0.5mm glass beads			X	X		X
VKMix	Micro-organism lysing	0.1mm & 0.5mm glass beads					X
SK38	Micro-organism lysing within tissue	0.1mm glass beads 1.4mm ceramic beads 1 glass bead of 4.0mm			X		X
CK01	Bacteria lysing	0.1mm ceramic beads			X		X	X
CKMix	Tissue homogenizing	1.4mm & 2.8mm ceramic beads	X		X
CK14	Soft tissue homogenizing	1.4mm ceramic beads		X	X�		X
CK28	Hard tissue homogenizing	2.8mm ceramic beads			X	X�
CKMix50	Tissue grinding	2.8mm & 5.0mm ceramic beads
CK68	Dry hard tissue grinding	6.8mm ceramic beads				X		X
MK28	Hard tissue grinding	2.8mm stainless steel beads			X
Metal tubes	Bones & teeth grinding	Empty tubes to fill with 6.8mm ceramic beads					X