[PRECELLYS] DNA Extraction from Chlorella sorokiniana (Algae)

Precellys®24 has been used by Promega internal application lab to extract gDNA from Chlorella sorokiniana, before sequencing (NGS). The efficiency of extraction with Precellys®24 has been compared to Proteinase K method based on heating treatment. DNA purification was performed using Promega Maxwell 16 equipment with the LEV Plant DNA kit.

• Precellys®24 (Bertin Technologies)
• Lysing Kit: VK05_2mL (KT03961-1-004.2)
• Sample: 50 mL of Chlorella sorokinianasuspension (overnight culture) / Several initial volumes of culture (2, 4, 5, 6 and 10 mL)
• Promega Maxwell 16 LEV Plant DNA Kit (AS1420)
• Promega Maxwell 16 Instrument (AS3000)
• NanoDrop 2000

• Centrifugation of each Chlorella sorokinianasuspension (2, 4, 5, 6 and 10 mL)
• Resuspension of the pellet in 500µL of TLA Buffer (Promega)
• Precellys® protocol: 6500 rpm, 2 cycles of 20s with 5s of break
• Heating parameters: 60 min / 70?
• DNA purification with Promega Maxwell 16 LEV Plant DNA Kit and Maxwell 16 Instrument
• Quantification of gDNA by NanoDrop
• Electrophoresis

DNA extraction yields obtained with Precellys®24 or with the Proteinase K method have been evaluated by NanoDrop quantification.

High  extraction  yields  are  obtained  with  Precellys ® 24 compared to the Proteinase K method. The ratios 260/280 and 260/230 are also very good with Precellys ® 24.

Thanks to the Precellys ® 24, high quality DNA  is extracted from Chlorella sorokiniana in high quantity.

Promega