[PRECELLYS] Protein extraction from cypress pollen with Minilys

The common cypress pollen (Cupressus sempervirens) is becoming an increasing cause of respiratory allergy in Europe and some regions worldwide. Because of its particular structural features and physico-chemical composition, cypress pollen is one of the most pollen difficult to analyze in terms of protein content and therefore allergens. In aqueous media, the external wall (exine) cracks in a few minutes under the effect of swelling of the intine (inner wall) particularly rich in polysaccharide. Few proteins are then extracted in aqueous conditions. The dry milling may be a good alternative for the extraction of cypress pollen proteins and to generate fragments of smaller sizes for experiments and ultrastructural analysis of immuno-reactivity [1].

[1] Y. Shahali, J.-P. Sutra, G. Peltre, D. Charpin, H. Sénéchal,P. Poncet. IgE reactivity to Common cypress (C. sempervirens)pollen extracts: evidence for novel allergens. WAO Journal.2010, 3, 229-234.